Preparation of Leukocyte-poor platelet concentrates via a short, hard spin of a pool of buffy coats.
van Delden CJ, Faber RD, de Wit HJ, Smit Sibinga CT.
Vox Sang. 2000;78(3):164-70.
Source
Blood Bank Noord Nederland, Groningen, The Netherlands.
Abstract
BACKGROUND AND OBJECTIVES :
A new method for the preparation of leukocyte-poor platelet concentrates was developed, based on a short, hard spin of a pool of 5 buffy coats (BCs) combined with automated collection of the platelets.
MATERIALS AND METHODS :
The characteristics of platelet concentrates (PCs) were studied as a function of the total g force applied to a pool of 5 BCs. Pools of BCs were centrifuged for 1 min with a total g force ranging from about 3,300 to 5,000 gmin (n = 7-9 per applied g force). Deceleration took place without the means of a brake. The total centrifugation time was about 11 min. The platelet-rich plasma (PRP) fraction above the cell layer was separated by an automated component preparation device.
RESULTS :
A short, hard spin with a total g force of between 3,400 and 4,600 gmin resulted in PCs that contained on average more than 290x10(9) Platelets and less than 5x10(6) Leukocytes without the use of a leukocyte filter, provided that the transfer of PRP was electronically checked and terminated. The cell concentrations in the PCs are a function of the total g force. Both the platelet and leukocyte levels in the concentrate decreased with an increase in the total g force applied to the pool.
CONCLUSION :
The preparation of PCs via a short hard, spin of BCs, combined with automated collection of the PRP, may be an alternative method for the preparation of leukocyte-poor PCs.
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