Platelet rich plasma Injection grafts for musculoskeletal injuries：a review（3）.
PRP preparation
Various blood separation devices have differing preparation steps essentially accomplishing similar goals. The Biomet Biologics GPS III system is described here for simplicity. About 30～60 ml of venous blood is drawn with aseptic technique from the anticubital vein. An 18 or 19 g butterfly needle is advised, in efforts of avoiding irritation and trauma to the platelets which are in a resting state. The blood is then placed in an FDA approved device and Centrifuged for 15 min at 3,200 rpm (Fig. 3). Afterward, the blood
is separated into platelet poor plasma (PPP), RBC, and PRP. Next the PPP is extracted through a special port and discarded from the device (Fig. 4). While the PRP is in a vacuumed space, the device is shaken for 30 s to re-suspend the platelets. Afterwards the PRP is withdrawn (Fig. 5). Depending on the initial blood draw, there is approximately 3 or 6 cc of PRP available.
Fig. 3
GPS III system and centrifuge
Fig. 4
GPS III system, withdrawing of platelet poor plasma to be discarded
Fig. 5
GPS III withdrawing of platelet rich plasma for injection/graft
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