Platelet-rich fibrin（PRF）：a second-generation platelet concentrate. Part III：Leucocyte activation：a new feature for platelet concentrates？
Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, Gogly B.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):e51-5.
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology Volume 101, Issue 3, March 2006, Pages e51–e55
Source
Biophysics Laboratory, Faculty of Dental Surgery, University of Paris V, Paris, France. drdohand@hotmail.com
http://www.sciencedirect.com/science/article/pii/S1079210405005883
Abstract
Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. In this third article, we investigate the immune features of this biomaterial. During PRF processing, Leucocytes could also secrete Cytokines in reaction to the Hemostatic and Inflammatory phenomena artificially induced in the centrifuged tube. We therefore undertook to quantify 5 significant cell mediators within platelet poor plasma supernatant and PRF clot exudate serum : 3 proinflammatory cytokines (IL-1beta, IL-6, and TNF-alpha), an antiinflammatory cytokine (IL-4), and a key growth promoter of angiogenesis (VEGF). Our data are correlated with that obtained in plasma (nonactivated blood) and in sera (activated blood). These initial analyses revealed that PRF could be an immune regulation node with inflammation retrocontrol abilities. This concept could explain the reduction of postoperative infections when PRF is used as surgical additive.
Fig. 1. Schematic representation of the 3 centrifugation strata obtained after PRF processing according to the official Process protocol.
Fig. 2. IL-1β ELISA Quantifications.
Fig. 3. IL-6 ELISA Quantifications.
Fig. 4. TNF-α ELISA Quantifications.
Fig. 5. IL-4 ELISA Quantifications.
Fig. 6. VEGF ELISA Quantifications.
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