察爾斯大夫 美麗殿堂 Dr. Charles Meridien Palace

Platelet-rich fibrin（PRF）：a second-generation platelet concentrate. Part V：histologic evaluations of PRF effects on bone allograft maturation in sinus lift.

Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, Dohan AJ, Mouhyi J, Dohan DM.

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):299-303.

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology Volume 101, Issue 3, March 2006, Pages 299–303

Source

Pain Clinic Center, Nice, France.

http://www.sciencedirect.com/science/article/pii/S1079210405005913

Abstract

OBJECTIVE:

Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling. The use of platelet gel to improve bone regeneration is a recent technique in implantology. However, the biologic properties and real effects of such products remain controversial. In this article, we therefore attempt to evaluate the potential of PRF in combination with freeze-dried bone allograft (FDBA) (Phoenix; TBF, France) to enhance bone regeneration in sinus floor elevation.

STUDY DESIGN:

Nine sinus floor augmentations were performed. In 6 sites, PRF was added to FDBA particles (test group), and in 3 sites FDBA without PRF was used (control group). Four months later for the test group and 8 months later for the control group, bone specimens were harvested from the augmented region during the implant insertion procedure. These specimens were treated for histologic analysis.

RESULTS:

Histologic evaluations reveal the presence of residual bone surrounded by newly formed bone and connective tissue. After 4 months of healing time, histologic maturation of the test group appears to be identical to that of the control group after a period of 8 months. Moreover, the quantities of newly formed bone were equivalent between the 2 protocols.

CONCLUSIONS:

Sinus floor augmentation with FDBA and PRF leads to a reduction of healing time prior to implant placement. From a histologic point of view, this healing time could be reduced to 4 months, but large-scale studies are still necessary to validate these first results.

Fig. 1. Preliminary analyses highlight mineralized trabecular bone rich in osteocytes which appear green (A and B) or blue (C and D) according to the staining. Osteoïd borders are stained in red (B and D) and are in contact with dense cellular osteoblast fronts. The richness of osteoïd tissue is evidence of important turnover in both types of samples (test and control).

Fig. 2. Mean histomorphometric analysis of bone samples from 3 sinus floor augmentations after a healing period of 8 months (control group: FDBA alone).

Fig. 3. Mean histomorphometric analysis of bone samples from 6 sinus floor augmentation after a healing period of 4 months (test group: FDBA+PRF).