Platelet-rich fibrin（PRF）：a second-generation platelet concentrate. Part I：technological concepts and evolution.
David M. Dohan, DDS, MSa, , , Joseph Choukroun, MDb, Antoine Diss, DDS, MSc, Steve L. Dohand, Anthony J.J. Dohane, Jaafar Mouhyi, DDS, PhDf, Bruno Gogly, DDS, MS, PhDg
a Assistant Professor, Biophysics Laboratory, Faculty of Dental Surgery, University of Paris V; Department of Oral Surgery, Odontology Service, Hopital Albert Chenevier, Paris
b Private Practice, Pain Clinic Center, Nice, France
c Assistant Professor, Laboratory of Surface and Interface in Odontology, Odontology Faculty, Nice University; Department of Periodontology, Odontology Service, Hopital St Roch, Nice, France
d Student, Biophysics Laboratory, Faculty of Dental Surgery, University of Paris V; Odontology Service, Hopital Albert Chenevier, Paris
e Student, Saint-Antoine Faculty of Medicine, University of Paris VI
f Private practice, Casablanca, Morocco; Assistant Professor, Advanced Periodontology, University of Southern California; Researcher, Department of Biomaterials/Handicap Research, Institute for Surgical Sciences, Sahlgrenska Academy at Göteborg University
g Professor, Faculty of Dental Surgery, University of Paris V; Chief, Odontology Service, Hopital Albert Chenevier, Paris
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar;101(3):e37-44. Epub 2006 Jan 19.
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
Volume 101, Issue 3, March 2006, Pages e37–e44
Source
Biophysics Laboratory, Faculty of Dental Surgery, University of Paris V, Paris, France. drdohand@hotmail.com
（http://www.sciencedirect.com/science/article/pii/S107921040500586X）
Abstract
Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates geared to simplified preparation without biochemical blood handling. In this initial article, we describe the conceptual and technical evolution from Fibrin glues to Platelet concentrates. This retrospective analysis is necessary for the understanding of fibrin technologies and the evaluation of the biochemical properties of 3 generations of surgical additives, respectively Fibrin adhesives, Concentrated Platelet-rich plasma (cPRP) and PRF. Indeed, the 3-dimensional fibrin architecture is deeply dependent on artificial clinical polymerization processes, such as massive bovine thrombin addition. Currently, the Slow polymerization during PRF preparation seems to generate a fibrin network very similar to the natural one. Such a network leads to a more efficient cell migration and proliferation and thus cicatrization.
Fig. 1. Technologic concept of cPRP processing
Fig. 2. Blood centrifugation immediately after collection allows the composition of a structured and resistant fibrin clot in the middle of the tube, just between the red corpuscles at the bottom and acellular plasma at the top.
Fig. 3. Blood processing with a PC-O2 centrifuge for PRF (A; Process, Nice, France) allows the composition of a structured fibrin clot in the middle of the tube, just between the red corpuscles at the bottom and acellular plasma at the top (B). After collection of the PRF itself (C), resistant autologous fibrin membranes are easily obtained by driving out the serum from the clot (D).
Fig. 4. Theoretical computer modelling of condensed tetramolecular or bilateral fibrin branch junctions. Note the rigidity of this architecture (D-TEP v1.3).
Fig. 5. Theoretical computer modelling of trimolecular or equilateral fibrin branch junctions. Note the flexibility of this net architecture (D-TEP v1.3).
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