PBMC：Peripheral blood mononucleated（mononuclear）cell
A peripheral blood mononucleated cell（PBMC）is any blood cell having a round nucleus（opposed to a lobed nucleus）. For example：a lymphocyte, a monocyte or a macrophage. These blood cells are a critical component in the immune system to fight infection and adapt to intruders.
The lymphocyte population consists of T cells（CD4 and CD8 positive～75%）, B cells and NK cells（～25% combined）.
These cells can be extracted from whole blood using Ficoll™, a hydrophilic polysaccharide that separates layers of blood, which will separate the blood into a top layer of plasma, followed by a layer of PBMCs and a bottom fraction of polymorphonuclear cells (such as neutrophils and eosinophils) and erythrocytes. The polymorphonuclear cells can be further isolated by lysing the red blood cells.
PBMCs are widely used in research and clinical applications.
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