Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation
Mahmood S Choudhery12, Michael Badowski2, Angela Muise2, John Pierce3 and David T Harris2*
* Corresponding author : David T Harris davidh@email.arizona.edu
Author Affiliations
1 Advanced Centre of Research in Biomedical Sciences, King Edward Medical University, Lahore, Pakistan
2 Department of Immunobiology, College of Medicine, The University of Arizona, PO Box 245221, 85724, Tucson, AZ, USA
3 Aesthetic Surgery of Tucson, Tucson, AZ, USA
Journal of Translational Medicine 2014, 12:8
doi:10.1186/1479-5876-12-8
The electronic version of this article is the complete one and can be found online at : http://www.translational-medicine.com/content/12/1/8
Received : 23 September 2013
Accepted : 3 December 2013
Published : 7 January 2014
© 2014 Choudhery et al.; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Abstract
Background
Human adipose tissue is an ideal autologous source of mesenchymal stem cells (MSCs) for various regenerative medicine and tissue engineering strategies. Aged patients are one of the primary target populations for many promising applications. It has long been known that advanced age is negatively correlated with an organism’s reparative and regenerative potential, but little and conflicting information is available about the effects of age on the quality of human adipose tissue derived MSCs (hAT-MSCs).
Methods
To study the influence of age, the expansion and in vitro differentiation potential of hAT-MSCs from young (<30 years), adult (35-50 years) and aged (>60 years) individuals were investigated. MSCs were characterized for expression of the genes p16INK4a and p21 along with measurements of population doublings (PD), superoxide dismutase (SOD) activity, cellular senescence and differentiation potential.
Results
Aged MSCs displayed senescent features when compared with cells isolated from young donors, concomitant with reduced viability and proliferation. These features were also associated with significantly reduced differentiation potential in aged MSCs compared to young MSCs.
Conclusions
In conclusion, advancing age negatively impacts stem cell function and such age related alterations may be detrimental for successful stem cell therapies.
Keywords : Adipose tissue; Mesenchymal stem cells; Donor age; Regenerative potential; Growth kinetics; In vitro differentiation potential
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