Classification of Platelet concentrates：from Pure platelet-rich plasma（P-PRP） to Leucocyte- and platelet-rich fibrin（L-PRF）（2）
Figure 2. Commonly described protocol for Anitua's PRGF 10, 11, 12, 13 and 14.
Step 1: Citrated blood in 5 mL tubes is softly centrifuged 8 min at 460 g and separated into three layers: RBC base, buffy coat (BC) and acellular plasma. Acellular plasma contains the empirically defined layers plasma poor in growth factors (PPGF) and plasma rich in growth factors (PRGF).
Step 2: The PPGF layer (1 ml) is discarded, and the PRGF, just above the BC, is collected by careful pipetting. PRGF from all sample tubes is collected into one tube and Calcium chloride is added for clotting.
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