Platelet-released growth factors enhance the secretion of Hyaluronic acid and induce Hepatocyte growth factor production by Synovial fibroblasts from arthritic patients
E. Anitua1, M. Sánchez2, A. T. Nurden3, M. M. Zalduendo1, M. de la Fuente1, J. Azofra2 and I. Andía1
1Biotechnology Institute, BTI IMASD, 2Unidad de Cirugia Artroscópica ‘Mikel Sanchez’, Vitoria, Spain and 3IFR4/FR21, Pessac, France.
Correspondence to : I. Andia, Biotechnology Institute, c/ Leonardo Da Vinci 14, 01510 Miñano, Alava, Spain. E-mail: isabel.andia@bti-imasd.com
Received May 31, 2007.
Revision received August 2, 2007.
Abstract
Objectives.
Autologous platelet-secreted growth factors (GFs) may have therapeutic effects in osteoarthritis (OA) capsular joints via multiple mechanisms. Our aim was to examine the effect of a platelet-derived preparation rich in growth factors (PRGFs) in OA synovial cell biology.
Methods.
Synovial cells were isolated from 10 osteoarthritic patients and cultured in serum-free media (basal conditions) and exposed to either a platelet-poor preparation or PRGF for 72 h. Cells activated with interleukin-1β (IL-1β) for 48 h were also exposed to PRGF. Changes in several events relevant to joint homeostasis including (i) hyaluronic acid (HA) secretion, (ii) the balance between metalloproteinase-1, -3 and -13 (MMP-1, MMP-3 and MMP-13) and tissue inhibitor-1 (TIMP-1) and (iii) the secretion of transforming growth factor-β1(TGF-β1), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF), were all assessed.
Results.
PRGF significantly enhanced HA secretion compared with platelet-poor preparations, P < 0.05; at the same time release of TIMP-1, MMP-1, MMP-3 and MMP-13 were not affected. An increased HGF production was observed (P < 0.05) but VEGF and TGF-β1 levels remained unchanged. PRGF significantly enhanced the secretion of HA induced by IL-1β activation, P < 0.05, but it did not modify the IL-1β-induced rise in MMP-1, MMP-3 and VEGF. In contrast, PRGF-induced HGF production was abolished by the presence of IL-1β during PRGF treatment, P < 0.05.
Conclusions.
Intra-articular administration of PRGF might be beneficial in restoring HA concentration and switching angiogenesis to a more balanced status but does not halt the effects of IL-1β on synovial cells.
Key words
Platelet-rich plasma Osteoarthritis Synovial cells IL-β Growth factors
© The Author 2007.
Published by Oxford University Press on behalf of the British Society for Rheumatology.
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