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Breaking down fat：Composition of Stromal vascular fraction

by Alexey Bersenev

on May 18, 2013

（http://stemcellassays.com/2013/05/breaking-fat-svf/）

In the previous post we defined adipose-derived stromal vascular fraction (SVF) versus stromal (stem) cells and highlighted difference between them. Today we will look at SVF more precisely and define its composition.

SVF is freshly isolated heterogeneous cell fraction, which could be derived from Native adipose tissue or Liposuction aspirates. SVF could be derived from both – the Fatty and Fluid portions of liposuction aspirates after enzymatic digestion. If SVF derived from fatty portion, it’s also called PLA（processed lipoaspirates）cells.

Basically, SVF is what remained in the pellet after removal blood and fat components. It is very crude and heterogeneous mix of multiple cell populations with different degree of maturity and function.

I draw a scheme of SVF isolation and composition：

Based on method of adipose tissue processing, cellular composition of SVF can vary significantly. Most sources indicate that Adipose-derived stromal（stem）cells represent up to 10% (2～10%) of SVF. Endothelial cells（mature and progenitors）could represents anything from 7% up to ～30% of SVF. Depending on processing, Fibroblasts could represent up to 50% of SVF (Cytori data, presented at ISCT 2010). CD34+ cells are present at large number and could compose up to 63% of SVF.

It has also been described that the SVF is composed of 11% CD2+ cells, 18% CD11a+ cells, 29% CD14+ cells, 49% CD31+ cells, 57% CD45+ cells, and 60% CD90+ cells（referring to ASCs and endothelial cells）. Others detected a different composition of the SVF（nearly 11% CD14+ cells, ~2% CD31+ cells, ~7% CD34+, ~9% CD45+ cells, ~29% CD90+, and ~47% 146+ cells）.

The SVF of human adipose tissue contained：Endothelial progenitors, (15.4 ± 4.8)% (mean ± standard error), Pericytes (2.0 ± 1.1)%, CD146+/CD34+ transitional cells 0.5 ± 0.3, and SA-ASC (59.0 ± 10.0)% of Non-hematopoietic （CD45−/CD14−/CD33−/glycophorin A−）singlet cells.

To summarize：

SVF composed of many Mature, Progenitor and Stem cell types. Depending on adipose tissue processing method, the composition of SVF and relative values of each cell population can vary significantly. Adipose-derived stromal（stem）cells represent 2～10% of SVF.